Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems
Identifieur interne : 002B84 ( Main/Exploration ); précédent : 002B83; suivant : 002B85Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems
Auteurs : Camila Ximenes [Brésil] ; Eduardo Brandão [Brésil] ; Paula Oliveira [Brésil] ; Abraham Rocha [Brésil] ; Tamisa Rego [Brésil] ; Rafael Medeiros [Brésil] ; Ana Aguiar-Santos [Brésil] ; João Ferraz [Brésil] ; Christian Reis [Brésil] ; Paulo Araujo [Brésil] ; Luiz Carvalho [Brésil] ; Fabio L. Melo [Brésil]Source :
- Memórias do Instituto Oswaldo Cruz [ 0074-0276 ] ; 2014.
Descripteurs français
- KwdFr :
- ADN des helminthes (isolement et purification), Adolescent, Adulte, Adulte d'âge moyen, Animaux, Antigènes de surface (sang), Antigènes de surface (urine), Femelle, Filariose lymphatique (diagnostic), Filariose lymphatique (sang), Filariose lymphatique (urine), Humains, Jeune adulte, Limite de détection, Microfilaria (génétique), Microfilaria (isolement et purification), Mâle, Réaction de polymérisation en chaîne (), Réaction de polymérisation en chaîne (normes), Sensibilité et spécificité, Wuchereria bancrofti (génétique), Wuchereria bancrofti (isolement et purification).
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : Microfilaria, Wuchereria bancrofti.
- isolement et purification : ADN des helminthes, Microfilaria, Wuchereria bancrofti.
- normes : Réaction de polymérisation en chaîne.
- sang : Antigènes de surface, Filariose lymphatique.
- urine : Adolescent, Adulte, Adulte d'âge moyen, Animaux, Antigènes de surface, Femelle, Filariose lymphatique, Humains, Jeune adulte, Limite de détection, Mâle, Réaction de polymérisation en chaîne, Sensibilité et spécificité.
English descriptors
- KwdEn :
- Adolescent, Adult, Animals, Antigens, Surface (blood), Antigens, Surface (urine), DNA, Helminth (isolation & purification), Elephantiasis, Filarial (blood), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (urine), Female, Humans, Limit of Detection, Male, Microfilariae (genetics), Microfilariae (isolation & purification), Middle Aged, Polymerase Chain Reaction (methods), Polymerase Chain Reaction (standards), Sensitivity and Specificity, Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification), Young Adult.
- MESH :
- chemical , blood : Antigens, Surface.
- chemical , isolation & purification : DNA, Helminth.
- chemical , urine : Antigens, Surface.
- blood : Elephantiasis, Filarial.
- diagnosis : Elephantiasis, Filarial.
- genetics : Microfilariae, Wuchereria bancrofti.
- isolation & purification : Microfilariae, Wuchereria bancrofti.
- methods : Polymerase Chain Reaction.
- standards : Polymerase Chain Reaction.
- urine : Elephantiasis, Filarial.
- Adolescent, Adult, Animals, Female, Humans, Limit of Detection, Male, Middle Aged, Sensitivity and Specificity, Young Adult.
Abstract
The Global Program for the Elimination of Lymphatic Filariasis (GPELF) aims to
eliminate this disease by the year 2020. However, the development of more specific
and sensitive tests is important for the success of the GPELF. The present study
aimed to standardise polymerase chain reaction (PCR)-based systems for the diagnosis
of filariasis in serum and urine. Twenty paired biological urine and serum samples
from individuals already known to be positive for
Url:
DOI: 10.1590/0074-0276140155
PubMed: 25424447
PubMed Central: 4325614
Affiliations:
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Luiz" uniqKey="Carvalho L" first="Luiz" last="Carvalho">Luiz Carvalho</name><affiliation wicri:level="1"><nlm:aff id="aff3">Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Melo, Fabio L" sort="Melo, Fabio L" uniqKey="Melo F" first="Fabio L" last="Melo">Fabio L. 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and urine samples using polymerase chain reaction-based systems</title><author><name sortKey="Ximenes, Camila" sort="Ximenes, Camila" uniqKey="Ximenes C" first="Camila" last="Ximenes">Camila Ximenes</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Brandao, Eduardo" sort="Brandao, Eduardo" uniqKey="Brandao E" first="Eduardo" last="Brandão">Eduardo Brandão</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Oliveira, Paula" sort="Oliveira, Paula" uniqKey="Oliveira P" first="Paula" last="Oliveira">Paula Oliveira</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Rocha, Abraham" sort="Rocha, Abraham" uniqKey="Rocha A" first="Abraham" last="Rocha">Abraham Rocha</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Rego, Tamisa" sort="Rego, Tamisa" uniqKey="Rego T" first="Tamisa" last="Rego">Tamisa Rego</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Medeiros, Rafael" sort="Medeiros, Rafael" uniqKey="Medeiros R" first="Rafael" last="Medeiros">Rafael Medeiros</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Aguiar Santos, Ana" sort="Aguiar Santos, Ana" uniqKey="Aguiar Santos A" first="Ana" last="Aguiar-Santos">Ana Aguiar-Santos</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ferraz, Joao" sort="Ferraz, Joao" uniqKey="Ferraz J" first="João" last="Ferraz">João Ferraz</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Reis, Christian" sort="Reis, Christian" uniqKey="Reis C" first="Christian" last="Reis">Christian Reis</name><affiliation wicri:level="1"><nlm:aff id="aff2">Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Araujo, Paulo" sort="Araujo, Paulo" uniqKey="Araujo P" first="Paulo" last="Araujo">Paulo Araujo</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Carvalho, Luiz" sort="Carvalho, Luiz" uniqKey="Carvalho L" first="Luiz" last="Carvalho">Luiz Carvalho</name><affiliation wicri:level="1"><nlm:aff id="aff3">Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Melo, Fabio L" sort="Melo, Fabio L" uniqKey="Melo F" first="Fabio L" last="Melo">Fabio L. Melo</name><affiliation wicri:level="1"><nlm:aff id="aff1">Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses, Centro de Pesquisas Aggeu Magalhães-Fiocruz, Recife, PE,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Memórias do Instituto Oswaldo Cruz</title><idno type="ISSN">0074-0276</idno><idno type="eISSN">1678-8060</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Animals</term><term>Antigens, Surface (blood)</term><term>Antigens, Surface (urine)</term><term>DNA, Helminth (isolation & purification)</term><term>Elephantiasis, Filarial (blood)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (urine)</term><term>Female</term><term>Humans</term><term>Limit of Detection</term><term>Male</term><term>Microfilariae (genetics)</term><term>Microfilariae (isolation & purification)</term><term>Middle Aged</term><term>Polymerase Chain Reaction (methods)</term><term>Polymerase Chain Reaction (standards)</term><term>Sensitivity and Specificity</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (isolation & purification)</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (isolement et purification)</term><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Antigènes de surface (sang)</term><term>Antigènes de surface (urine)</term><term>Femelle</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (sang)</term><term>Filariose lymphatique (urine)</term><term>Humains</term><term>Jeune adulte</term><term>Limite de détection</term><term>Microfilaria (génétique)</term><term>Microfilaria (isolement et purification)</term><term>Mâle</term><term>Réaction de polymérisation en chaîne ()</term><term>Réaction de polymérisation en chaîne (normes)</term><term>Sensibilité et spécificité</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Surface</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="urine" xml:lang="en"><term>Antigens, Surface</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Microfilariae</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Microfilaria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Microfilariae</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN des helminthes</term><term>Microfilaria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes de surface</term><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="urine" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Animals</term><term>Female</term><term>Humans</term><term>Limit of Detection</term><term>Male</term><term>Middle Aged</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" qualifier="urine" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Antigènes de surface</term><term>Femelle</term><term>Filariose lymphatique</term><term>Humains</term><term>Jeune adulte</term><term>Limite de détection</term><term>Mâle</term><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The Global Program for the Elimination of Lymphatic Filariasis (GPELF) aims to
eliminate this disease by the year 2020. However, the development of more specific
and sensitive tests is important for the success of the GPELF. The present study
aimed to standardise polymerase chain reaction (PCR)-based systems for the diagnosis
of filariasis in serum and urine. Twenty paired biological urine and serum samples
from individuals already known to be positive for<italic> Wuchereria bancrofti
</italic>were collected during the day. Conventional PCR and semi-nested PCR assays
were optimised. The detection limit of the technique for purified <italic>W.
bancrofti </italic>DNA extracted from adult worms was 10 fg for the internal
systems (WbF/Wb2) and 0.1 fg by using semi-nested PCR. The specificity of the primers
was confirmed experimentally by amplification of 1 ng of purified genomic DNA from
other species of parasites. Evaluation of the paired urine and serum samples by the
semi-nested PCR technique indicated only two of the 20 tested individuals were
positive, whereas the simple internal PCR system (WbF/Wb2), which has highly
promising performance, revealed that all the patients were positive using both
samples. This study successfully demonstrated the possibility of using the PCR
technique on urine for the diagnosis of <italic>W. bancrofti </italic>infection.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Abbasi, I" uniqKey="Abbasi I">I Abbasi</name></author><author><name sortKey="Githure, J" uniqKey="Githure J">J Githure</name></author><author><name sortKey="Ochola, Jj" uniqKey="Ochola J">JJ Ochola</name></author><author><name sortKey="Agure, R" uniqKey="Agure R">R Agure</name></author><author><name sortKey="Koech, Dk" uniqKey="Koech D">DK Koech</name></author><author><name sortKey="Ramzy, Rm" uniqKey="Ramzy R">RM Ramzy</name></author><author><name sortKey="Williams, Sa" uniqKey="Williams S">SA Williams</name></author><author><name sortKey="Hamburger, J" uniqKey="Hamburger J">J Hamburger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Amaral, F" uniqKey="Amaral F">F Amaral</name></author><author><name sortKey="Dreyer, G" uniqKey="Dreyer G">G 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Chanteau</name></author><author><name sortKey="Nutman, Tb" uniqKey="Nutman T">TB Nutman</name></author><author><name sortKey="Ottesen, Ea" uniqKey="Ottesen E">EA Ottesen</name></author><author><name sortKey="Williams, Sa" uniqKey="Williams S">SA Williams</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Brésil</li></country></list><tree><country name="Brésil"><noRegion><name sortKey="Ximenes, Camila" sort="Ximenes, Camila" uniqKey="Ximenes C" first="Camila" last="Ximenes">Camila Ximenes</name></noRegion><name sortKey="Aguiar Santos, Ana" sort="Aguiar Santos, Ana" uniqKey="Aguiar Santos A" first="Ana" last="Aguiar-Santos">Ana Aguiar-Santos</name><name sortKey="Araujo, Paulo" sort="Araujo, Paulo" uniqKey="Araujo P" first="Paulo" last="Araujo">Paulo Araujo</name><name sortKey="Brandao, Eduardo" sort="Brandao, Eduardo" uniqKey="Brandao E" first="Eduardo" last="Brandão">Eduardo Brandão</name><name sortKey="Carvalho, Luiz" sort="Carvalho, Luiz" uniqKey="Carvalho L" first="Luiz" last="Carvalho">Luiz Carvalho</name><name sortKey="Ferraz, Joao" sort="Ferraz, Joao" uniqKey="Ferraz J" first="João" last="Ferraz">João Ferraz</name><name sortKey="Medeiros, Rafael" sort="Medeiros, Rafael" uniqKey="Medeiros R" first="Rafael" last="Medeiros">Rafael Medeiros</name><name sortKey="Melo, Fabio L" sort="Melo, Fabio L" uniqKey="Melo F" first="Fabio L" last="Melo">Fabio L. Melo</name><name sortKey="Oliveira, Paula" sort="Oliveira, Paula" uniqKey="Oliveira P" first="Paula" last="Oliveira">Paula Oliveira</name><name sortKey="Rego, Tamisa" sort="Rego, Tamisa" uniqKey="Rego T" first="Tamisa" last="Rego">Tamisa Rego</name><name sortKey="Reis, Christian" sort="Reis, Christian" uniqKey="Reis C" first="Christian" last="Reis">Christian Reis</name><name sortKey="Rocha, Abraham" sort="Rocha, Abraham" uniqKey="Rocha A" first="Abraham" last="Rocha">Abraham Rocha</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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